In this paper we present a polynomial time algorithm, that provably calculates the exact values of MBD for the digital images. We compare this new algorithm, theoretically and experimentally, with the algorithm presented in , which computes the approximate values of the MBD. Moreover, we notice that every generalized distance function can be naturally translated to an image segmentation algorithm. The algorithms that fall under such category include: Relative Fuzzy Connectedness, and those associated with the minimum barrier, fuzzy distance, and geodesic distance functions. In particular, we compare experimentally these four algorithms on the 2D and 3D natural and medical images with known ground truth and at varying level of noise, blur, and inhomogeneity.
Materials and Methods: Six healthy adult male dogs were scanned twice, at weeks 0 (baseline) and 4, of the dietary regiment. FWMRI-derived volumes of adipose tissue (total, visceral, and subcutaneous), lean tissue, and cortical bone were quantified using a semi-automated approach. Volumes were converted to masses using published tissue densities.
Results: FWMRI-derived total mass corresponds with scale mass with a concordance correlation coefficient of 0.931 (95 confidence interval = [0.813, 0.975]), and slope and intercept values of 1.12 and -2.23 kg, respectively. Visceral, subcutaneous and total adipose tissue masses increased significantly from weeks 0 to 4, while neither cortical bone nor lean tissue masses changed significantly. This is evidenced by a mean percent change of 70.2 for visceral, 67.0 for subcutaneous, and 67.1 for total adipose tissue.
Conclusion: FWMRI can precisely quantify and map body composition with respect to adipose, lean, and bone tissue depots. The described approach provides a valuable tool to examine the role of distinct tissue depots in an established animal model of human metabolic disease
Here we present a radical improvement in oil red O worm staining together with high-throughput image-based phenotyping. The three-step sample preparation method is robust, formaldehyde-free, and inexpensive, and requires only 15 min of hands-on time to process a 96-well plate. Together with our free and user-friendly automated image analysis package, this method enables C. elegans sample preparation and phenotype scoring at a scale that is compatible with genome-wide screens. Thus we present a feasible approach to small-scale phenotyping and large-scale screening for genetic and/or chemical perturbations that lead to alterations in fat quantity and distribution in whole animals.