The research processes involves growing a huge amount of neurons in the laboratories and keeping track of the growth-rate and growth-behavior of these cells. There can be thousands of cells to keep track of every week. To perform these quantitative assessments of the growing cells the researchers started measuring the lengths of the neurite outgrowths, growing out from the seed of the cells.
In this master thesis we have developed a digital image processing computer software for extracting and measuring neurite outgrowths in digital images.
The processing of one such image involves four main digital image processing fields; these are thresholding, object classification, morphological operations and measuring by skeletonizing.
Three experiments have been done to test the final program. The test images were from rats and a Marmoset monkey. The experiments showed that a method developed for human brain images can be used for full body registrations of animal images with a satisfying result, especially if the images are from the same animal. When the images are from different individuals the results are a little poorer, but still fairly good.