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Presentation Information     2012-12-14 (14:00)   •  The seminar room at Vi2

Speaker Mike Hawralycz
Comment The Allen Institute for Brain Science, Seattle, USA
Type External presentation
Allen Mouse Brain Connectivity Atlas mapping long-range axonal projections from genetically-defined cell populations in the adult mouse brain
A comprehensive description of the connections of functional circuits in the mouse brain will provide an anatomical framework for understanding higher order cognitive processes and diseases that affect these networks. We have established a public online database, the Allen Mouse Brain Connectivity Atlas, a 3-D atlas of inter-areal neural projections across the adult mouse brain based on imaging axons fluorescently labeled with rAAVs by serial two-photon tomography. The current Atlas consists of projection mapping from ~300 anatomically-defined regions sampled from all major brain structures in cortical and subcortical areas. This strictly anatomical-based projection map is complemented by a larger-scale effort to map projections from genetically-identified cell populations throughout the brain using over 100 Cre driver mice and rAAVs expressing Cre-dependent fluorescent proteins. Cre lines are systematically characterized through ISH profiling of reporter genes, and those with anatomically-restricted and/or cell type-specific Cre expression in subcortical areas, and with laminar-selective expression of Cre in cortical areas are included in this regional and cell type-selective projection map. We have established an informatics pipeline to register these large datasets of images into a common 3-D space and to build 3-D axonal projection models which will facilitate search and comparison between this Atlas and other resources. Initial analyses indicate that our data are consistent with known projections from well-studied areas and cell types, but also provides a level of resolution and completeness throughout the entire mouse brain that can reveal novel connections between even very distal areas and other under-studied pathways. All data are freely available via the Allen Brain Atlas portal at brain-map.org