||The technique of whole embryo culture of rodents (where rat and mouse embryos can be cultured outside the uterus) has been used for over four decades to study adverse effects of chemicals and drugs on embryonic development. A major advantage of whole embryo culture is that development can be directly monitored during organogenesis. For instance the presence of heart rate and blood circulation, a sign of a healthy embryo, is normally monitored at different times during culture. Usually this is done manually under a light microscope where the culture flask is placed and the heart rate is determine by simply counting the number of heart beats for period of time.
We propose a new method which determines the heart location in the mouse embryo, and then calculate the heart beat automatically. We are provided with time frame sequences of mouse embryos. We have approached this problem from frequency domain point of view, and have exploited the information provided by the sampling rate of the time frame sequence to detect the heart location. Then cross-correlation is used as a similarity measure to determine the heart rate.